3B9J

Structure of Xanthine Oxidase with 2-hydroxy-6-methylpurine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	133.187, 73.794, 146.498
Unit cell angles	90.00, 98.87, 90.00

Refinement procedure

Resolution	144.340 - 2.300
R-factor	0.197
Rwork	0.194
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fiq
RMSD bond length	0.017
RMSD bond angle	1.798
Data scaling software	At
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	144.340	2.360
High resolution limit [Å]	2.300	2.300
Number of reflections	114520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	batch	7	298	PEG 8000, DTT, pyrophosphate, phosphate, TRIS, pH 7.0, batch, temperature 298K