3B9I
Crystal Structure of mouse GITRL at 2.5 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.791, 70.024, 71.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.490 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24800 |
| Starting model (for MR) | 2qdn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.326 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.140 | 0.525 |
| Number of reflections | 9637 | |
| <I/σ(I)> | 5.9 | 3.2 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 5.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 30% Pentaerythritol Ethoxylate(15/4 EO/OH), 0.05M Ammonium Sulfate and 0.05M Bis-Tris (pH 6.5), Vapor diffusion, Sitting drop, temperature 298K |
