3B9G
Crystal structure of loop deletion mutant of Trypanosoma vivax nucleoside hydrolase (3GTvNH) in complex with ImmH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8148 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.177, 74.881, 72.424 |
| Unit cell angles | 90.00, 98.07, 90.00 |
Refinement procedure
| Resolution | 40.320 - 1.400 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hoz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.252 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.320 | 1.436 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.072 | 0.605 |
| Number of reflections | 112485 | |
| <I/σ(I)> | 10.98 | 2.27 |
| Completeness [%] | 99.9 | 99.73 |
| Redundancy | 4.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 20% PEG 4000, 10mM sodium acetate, 0.25M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
