3B95
EuHMT1 (Glp) Ankyrin Repeat Domain (Structure 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.520, 152.390, 167.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.910 - 2.990 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB: 3B7B |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.910 | 3.100 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.150 | 0.697 |
| Number of reflections | 15898 | |
| <I/σ(I)> | 9 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 1.5-2.0 M Lithium Sulfate; 1-4% Peg 400 or 550; 0.1 M Tris buffer pH=8.5 , VAPOR DIFFUSION, SITTING DROP, temperature 289K |
