3B8S
Crystal structure of wild-type chitinase A from Vibrio harveyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.267, 64.283, 83.522 |
| Unit cell angles | 91.74, 91.18, 112.91 |
Refinement procedure
| Resolution | 24.620 - 2.000 |
| R-factor | 0.17022 |
| Rwork | 0.168 |
| R-free | 0.20510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b9e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.968 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.620 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.228 |
| Number of reflections | 73693 | |
| <I/σ(I)> | 19.5 | 5.5 |
| Completeness [%] | 94.6 | 91.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | 1.20M Ammonium Sulfate, 0.1M Tris-HCl pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
