3B8O
Structure of WzzE- Bacterial Polysaccharide Co-polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.438, 108.299, 122.569 |
| Unit cell angles | 90.00, 102.15, 90.00 |
Refinement procedure
| Resolution | 47.890 - 2.400 |
| R-factor | 0.22546 |
| Rwork | 0.223 |
| R-free | 0.26562 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.365 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.083 | 0.297 |
| Number of reflections | 117899 | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 96.8 | 78.8 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | PEG 3350, pH 7.0, vapor diffusion, temperature 298K, pH 7.00 |
