3B8J
Q191A mutant of DegS-deltaPDZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 |
| Unit cell lengths | 70.892, 70.892, 120.837 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.367 - 2.507 |
| Rwork | 0.234 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.720 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.058 | 0.163 |
| Number of reflections | 6682 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 85.9 | 54 |
| Redundancy | 7.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50 mM Na Cacodylate, 150 mM NaCitrate, 18% isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
