3B88

Complex of T57A Substituted Drosophila LUSH Protein with Ethanol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Detector technology	CCD
Collection date	2005-11-15
Detector	NOIR-1
Wavelength(s)	1.06
Spacegroup name	C 2 2 21
Unit cell lengths	65.118, 67.284, 110.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.850 - 2.000
R-factor	0.199
Rwork	0.195
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.053
Data reduction software	d*TREK
Data scaling software	d*TREK (9.3L)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.850	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.068	0.337
Total number of observations		11157
Number of reflections	16772
<I/σ(I)>	15	5
Completeness [%]	99.8	99.6
Redundancy	6.88	6.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1