3B85
Crystal structure of predicted phosphate starvation-induced ATPase PhoH2 from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 3 |
| Unit cell lengths | 98.668, 98.668, 36.675 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.690 - 2.350 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.569 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.098 | 0.448 |
| Number of reflections | 15693 | |
| <I/σ(I)> | 7.6 | 1.8 |
| Completeness [%] | 93.9 | 72.8 |
| Redundancy | 5.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 1.26M Ammonium sulfate, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
