3B7Q
Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.435, 71.727, 115.235 |
| Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
| Resolution | 40.630 - 2.030 |
| Rwork | 0.204 |
| R-free | 0.24100 |
| Starting model (for MR) | 3b74 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 4.370 | 2.030 |
| Rmerge | 0.086 | 0.056 | 0.289 |
| Number of reflections | 50846 | ||
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 95.8 | 90.6 | 93.2 |
| Redundancy | 4.1 | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 15-25% PEG 3350, 5% glycerol, and either 100 mM ammonium sulfate or 100mM potassium phosphate, 100 mM ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
