3B7Q

Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylcholine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	49.435, 71.727, 115.235
Unit cell angles	90.00, 94.08, 90.00

Refinement procedure

Resolution	40.630 - 2.030
Rwork	0.204
R-free	0.24100
Starting model (for MR)	3b74
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.100
High resolution limit [Å]	2.030	4.370	2.030
Rmerge	0.086	0.056	0.289
Number of reflections	50846
<I/σ(I)>	14.2
Completeness [%]	95.8	90.6	93.2
Redundancy	4.1	4.2	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	15-25% PEG 3350, 5% glycerol, and either 100 mM ammonium sulfate or 100mM potassium phosphate, 100 mM ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K