3B7P
Crystal structure of spermidine synthase from Plasmodium falciparum in complex with spermine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 199.483, 134.584, 48.370 |
| Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
| Resolution | 29.440 - 2.000 |
| R-factor | 0.21634 |
| Rwork | 0.215 |
| R-free | 0.24978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pwp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.325 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 84798 | |
| <I/σ(I)> | 16.67 | 2.21 |
| Completeness [%] | 98.5 | 90.8 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 291 | 25% w/v PEG 3350, 0.1M Ammonium sulfate, 0.1M Bis-tris, pH 5.5, VAPOR DIFFUSION, temperature 291K |
