3B7H
Crystal structure of the prophage Lp1 protein 11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.951, 65.650, 27.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.510 - 2.000 |
| R-factor | 0.19816 |
| Rwork | 0.196 |
| R-free | 0.24945 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.497 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.510 | 2.053 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.282 |
| Number of reflections | 5218 | |
| <I/σ(I)> | 32.74 | 4.85 |
| Completeness [%] | 96.3 | 79.75 |
| Redundancy | 8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 289 | 0.1M Na/K Phosphate pH 6.2, 10% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
