3B78
Structure of the eEF2-ExoA(R551H)-NAD+ complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 327.136, 68.128, 190.577 |
Unit cell angles | 90.00, 102.99, 90.00 |
Refinement procedure
Resolution | 46.550 - 2.500 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.24200 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.591 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.176 | 0.505 |
Number of reflections | 134457 | |
<I/σ(I)> | 6.7 | 1.3 |
Completeness [%] | 93.1 | 72.1 |
Redundancy | 3.5 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |