3B73
Crystal structure of the PhiH1 repressor-like protein from Haloarcula marismortui
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.392, 53.483, 89.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.290 - 2.120 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.178 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.290 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 12752 | |
| <I/σ(I)> | 11.5 | 5.4 |
| Completeness [%] | 90.5 | 64.7 |
| Redundancy | 13.8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 289 | 1.26M Ammonium sulfate, 0.1M Acetate pH 4.5, 0.2M Sodium chloride, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
