3B6O
Structure of TREX1 in complex with a nucleotide and an inhibitor ion (lithium)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.619, 81.337, 92.706 |
| Unit cell angles | 90.00, 103.07, 90.00 |
Refinement procedure
| Resolution | 24.670 - 2.100 |
| R-factor | 0.21508 |
| Rwork | 0.213 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2o4g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.056 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 65149 | |
| <I/σ(I)> | 5.9 | 1.5 |
| Completeness [%] | 93.9 | 75.6 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 22% PEG3350, 100 mM MES pH 6.0, 200 mM Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
