3B5X

Crystal Structure of MsbA from Vibrio cholerae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0077, 1.0094
Spacegroup name	P 21 21 21
Unit cell lengths	120.590, 150.420, 148.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.970 - 5.500
Rwork	0.348
R-free	0.36000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	5.500
Number of reflections	7570
Completeness [%]	84.0
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.4	277	100-120mM NaCl, 16.5-20% PEG 400 and 0.05% beta-DDM, pH 9.4, sitting drop vapor diffusion, temperature 277K