3B4S
Crystal structure of a LuxT domain from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935, 0.97948 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 147.438, 147.438, 382.487 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.220 - 3.100 |
| R-factor | 0.19237 |
| Rwork | 0.190 |
| R-free | 0.23218 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.802 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 3.190 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.123 | 0.756 |
| Number of reflections | 29395 | |
| <I/σ(I)> | 23.3 | 2.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M Bis-tris propane, 1.3M di-Ammonium tartrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
