3B4O
Crystal structure of phenazine biosynthesis protein PhzA/B from Burkholderia cepacia R18194, apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.660, 64.660, 160.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.690 - 1.900 |
R-factor | 0.188 |
Rwork | 0.186 |
R-free | 0.24500 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.686 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.059 | 0.374 |
Number of reflections | 31421 | |
<I/σ(I)> | 19.3 | 3.9 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 8.3 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 285 | 16 20% (w/v) PEG 3350, 0.2 M NH4-acetate, 0.1 M Bis-TRIS pH 6.1 6.7, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K |