3B44
Crystal structure of GlpG W136A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 1.0809 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 110.700, 110.700, 127.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| Rwork | 0.200 |
| R-free | 0.21700 |
| Starting model (for MR) | 2ic8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.064 | 0.284 |
| Number of reflections | 33056 | |
| <I/σ(I)> | 17.2 | 4.1 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | 3.5M NH4Cl, 100mM Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
