3B43
I-band fragment I65-I70 from titin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.430, 141.430, 166.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.985 - 3.300 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26700 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.944 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 17.000 | 3.350 | |
| High resolution limit [Å] | 3.300 | 16.000 | 3.300 |
| Rmerge | 0.080 | 0.019 | 0.478 |
| Number of reflections | 14953 | 25 | 639 |
| <I/σ(I)> | 19.6 | 54 | 4.3 |
| Completeness [%] | 98.6 | 13.8 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 298 | pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
