3B42
Periplasmic sensor domain of chemotaxis protein GSU0935
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-27 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.720, 70.740, 81.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.390 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.350 |
| Number of reflections | 38984 | |
| <I/σ(I)> | 28.9 | 5.6 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 8.2 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | PEG-Ion screen: 6, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
