3B3L
Crystal structures of alternatively-spliced isoforms of human ketohexokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.555, 140.704, 179.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.380 - 2.900 |
| R-factor | 0.23843 |
| Rwork | 0.236 |
| R-free | 0.28074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hqq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.243 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.432 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.060 | 0.354 |
| Number of reflections | 49962 | |
| <I/σ(I)> | 6.8 | 3.6 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 20% PEG 3000, 0.1M sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
