3B3J
The 2.55 A crystal structure of the apo catalytic domain of coactivator-associated arginine methyl transferase I(CARM1:28-507, residues 28-146 and 479-507 not ordered)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 136.022, 136.022, 125.304 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 117.850 - 2.550 |
| R-factor | 0.23674 |
| Rwork | 0.235 |
| R-free | 0.26885 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.532 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.850 | 2.630 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.064 | 0.282 |
| Number of reflections | 21499 | |
| <I/σ(I)> | 25.9 | 6.1 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 11.6 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 5% MPD, 100mM sodium citrate, 100 mM NaCl, 100 mM benzamidine chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
