3B2P
Crystal structure of E. coli Aminopeptidase N in complex with arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.204, 120.204, 169.655 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.370 - 2.000 |
| R-factor | 0.15448 |
| Rwork | 0.153 |
| R-free | 0.18846 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hpo |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.570 |
| Number of reflections | 95605 | |
| <I/σ(I)> | 13.2 | 2.4 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 5.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.8 M Sodium malonate pH=7/0, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
