3B2M
Crystal Structure of the Major Pilin from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.309, 52.308, 128.053 |
| Unit cell angles | 90.00, 97.33, 90.00 |
Refinement procedure
| Resolution | 66.800 - 2.220 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26400 |
| Structure solution method | SIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.800 | 2.300 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.070 | 0.682 |
| Number of reflections | 42842 | |
| <I/σ(I)> | 13.8 | 65.2 |
| Completeness [%] | 97.5 | 92.4 |
| Redundancy | 9.6 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2M sodium formate, 20% PEG 3350, 0.2M HEPES-HCl pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
