3B1T
Crystal structure of human peptidylarginine deiminase 4 in complex with o-Cl-amidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.787, 60.853, 114.302 |
Unit cell angles | 90.00, 123.99, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.2138 |
Rwork | 0.211 |
R-free | 0.26290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wda |
RMSD bond length | 0.013 |
RMSD bond angle | 1.484 |
Refinement software | REFMAC (5.6.0085) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 27393 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |