3B0Z

Crystal structure of cytoplasmic domain of FlhB from Salmonella typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-15
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 42 21 2
Unit cell lengths	49.102, 49.102, 143.045
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.070 - 2.450
R-factor	0.23163
Rwork	0.231
R-free	0.24709
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	2.090
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.450	2.580
High resolution limit [Å]	2.450	2.450
Number of reflections	6875
Completeness [%]		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1M sodium cacodylate, 0.2M zinc acetate, 25% propylene glycol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
2