3AZV

Crystal structure of the receptor binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-24
Detector	RAYONIX MX225HE
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	57.960, 138.570, 162.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.460 - 3.100
R-factor	0.25155
Rwork	0.250
R-free	0.28633
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.6.0091)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	3.270
High resolution limit [Å]	3.100	3.100
Number of reflections	45646
Completeness [%]	99.4	99.9
Redundancy		5.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4	293	0.1M MES, 1.6M magnesium sulfate, 1M sodium chloride, pH 6.4, VAPOR DIFFUSION, temperature 293K