3AZD
Crystal structure of tropomyosin N-terminal fragment at 0.98A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 31 |
| Unit cell lengths | 33.000, 33.000, 52.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.290 - 0.980 |
| R-factor | 0.16753 |
| Rwork | 0.167 |
| R-free | 0.17084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1IHQ model1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.415 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.730 | 1.030 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Number of reflections | 34162 | |
| Completeness [%] | 98.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.17M ammonium sulfate, 10% PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
