3AZ5
Crystal structure of Pt/O-HEWL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.06440 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.144, 55.006, 71.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.500 - 2.340 |
| R-factor | 0.23246 |
| Rwork | 0.229 |
| R-free | 0.30292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bgi |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (6.1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.380 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.052 | |
| Number of reflections | 5324 | |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 303 | sodium chloride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 30K, temperature 303K |
