3AYS
GH5 endoglucanase from a ruminal fungus in complex with cellotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.204, 82.204, 221.856 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.16427 |
| Rwork | 0.162 |
| R-free | 0.21826 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ayr |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.913 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.076 | 0.619 |
| Number of reflections | 22798 | |
| <I/σ(I)> | 60.7 | 8.3 |
| Completeness [%] | 97.0 | 97.3 |
| Redundancy | 23.1 | 25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1.5M sodium citrate, 6mM cellotetraose, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
