3AYL
X-ray crystal structures of L-phenylalanine oxidase (deaminating and decaboxylating) from Pseudomonas sp. P501. Structures of the enzyme-ligand complex and catalytic mechanism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-14 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.269, 112.843, 136.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.270 - 1.250 |
| R-factor | 0.101 |
| Rwork | 0.099 |
| R-free | 0.12800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yr4 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.298 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.270 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.077 | 0.347 |
| Number of reflections | 415723 | |
| Completeness [%] | 97.4 | 98.4 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 297 | 0.1M HEPES PH 7.5, 1.0M AMMONIUM SULFATE, VAPOR DIFFUSION, temperature 297K |
