3AYH
Crystal structure of the C17/25 subcomplex from S. pombe RNA Polymerase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Detector technology | CCD |
| Collection date | 2007-05-31 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.400, 85.400, 93.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.376 - 2.193 |
| R-factor | 0.1927 |
| Rwork | 0.190 |
| R-free | 0.23650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ckz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.002 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.380 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.108 | 0.669 |
| Number of reflections | 20649 | |
| <I/σ(I)> | 6.837 | 4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.7 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.3M Li2SO4, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
