3AY7
Crystal structure of Bacillus megaterium glucose dehydrogenase 4 G259A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.116, 128.260, 64.641 |
| Unit cell angles | 90.00, 110.73, 90.00 |
Refinement procedure
| Resolution | 30.450 - 1.900 |
| R-factor | 0.20201 |
| Rwork | 0.200 |
| R-free | 0.24583 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.136 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.441 |
| Number of reflections | 38880 | |
| <I/σ(I)> | 3.67 | 3.67 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M sodium cacodylate, 0.2M magnesium chloride, 50.5% PEG 200, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
