3AY3

Crystal structure of glucuronic acid dehydrogeanse from Chromohalobacter salexigens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-20
Detector	ADSC QUANTUM 210
Wavelength(s)	1.23985
Spacegroup name	P 63
Unit cell lengths	122.762, 122.762, 150.476
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.2184
Rwork	0.215
R-free	0.28860
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.784
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	RESOLVE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	79652
Completeness [%]	97.8	88.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	PEG 8000, Ca(C2H3O2)2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	8	295	PEG 8000, Ca(C2H3O2)2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K