3AXH
Crystal structure of isomaltase in complex with isomaltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.892, 114.736, 61.496 |
| Unit cell angles | 90.00, 90.99, 90.00 |
Refinement procedure
| Resolution | 24.380 - 1.800 |
| R-factor | 0.16927 |
| Rwork | 0.168 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aj7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.530 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.380 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.210 |
| Number of reflections | 56198 | |
| <I/σ(I)> | 12.7 | 5.8 |
| Completeness [%] | 97.1 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 288 | 50mM HEPES pH 7.3, 0.2M lithium acetate, 21% (w/v) PEG 3350, 0.2M isomaltose , VAPOR DIFFUSION, HANGING DROP, temperature 288K |
