3AXG
Structure of 6-aminohexanoate-oligomer hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 155.863, 214.446, 478.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.990 - 2.000 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.111 | 0.254 |
| Number of reflections | 501977 | |
| <I/σ(I)> | 10.6 | 2.32 |
| Completeness [%] | 94.3 | 73 |
| Redundancy | 3.6 | 2.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 283 | 1.0M SODIUM CITRATE, 0.2M SODIUM CHLORIDE, 0.1M HEPES BUFFER(PH 7.5), VAPOR DIFFUSION, SITTING DROP, temperature 283K |
