3AX3
Crystal structure of rat TOM20-ALDH presequence complex: a complex (form2) between Tom20 and a disulfide-bridged presequence peptide containing D-Cys and L-Cys at the i and i+3 positions.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2008-06-25 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.00 |
Spacegroup name | H 3 2 |
Unit cell lengths | 99.596, 99.596, 195.180 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.940 - 2.100 |
R-factor | 0.2452 |
Rwork | 0.243 |
R-free | 0.28698 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1WT4 2v1t |
RMSD bond length | 0.024 |
RMSD bond angle | 2.006 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (MR) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.045 | 0.496 | |
Number of reflections | 22101 | ||
Completeness [%] | 99.7 | 97 | 100 |
Redundancy | 9.3 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl pH8.0, 0.2M MgCl2, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |