3AX1
Molecular insights into miRNA processing by Arabidopsis Serrate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2814, 1.2818, 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.965, 80.663, 112.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.190 - 2.740 |
| R-factor | 0.24504 |
| Rwork | 0.243 |
| R-free | 0.28608 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.073 | 0.476 |
| Number of reflections | 11847 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG4K, Potassium formate, Tris, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
