3AWR
Crystal structure of Rat TOM20-ALDH presequence complex: The intermolecular disulfide bond was cleaved in the crystal of a disulfide-tethered complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-07-12 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.770, 27.723, 70.812 |
| Unit cell angles | 90.00, 102.71, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.20813 |
| Rwork | 0.206 |
| R-free | 0.25073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1t |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.421 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (MR) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.010 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.089 | 0.072 | 0.296 |
| Number of reflections | 8725 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 98.3 | 99.6 | 89.1 |
| Redundancy | 3.4 | 3.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.09M MES pH6.0, 0.045M MgCl2, 24% PEG 3350, 10mM DTT , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
