3AWN

Crystal structure of D-serine dehydratase from chicken kidney (EDTA treated)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-03-06
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 4 2 2
Unit cell lengths	105.329, 105.329, 82.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.21293
Rwork	0.211
R-free	0.25677
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3anu
RMSD bond length	0.011
RMSD bond angle	1.433
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.4.0078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	11883
<I/σ(I)>	12.6
Completeness [%]	99.9	99.7
Redundancy	6.9	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOUR DIFFUSION, HANGING DROP, MICRO-SEEDING	6.5	293	12-15% PEG 4000, 50mM MES-NaOH, 10% 2-propanol, pH 6.5, VAPOUR DIFFUSION, HANGING DROP, MICRO-SEEDING, temperature 293K