3AW6
Crystal structure of tetragonal hen egg white lysozyme at 84.2% relative humidity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.484, 79.484, 37.886 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.060 - 2.100 |
| R-factor | 0.15783 |
| Rwork | 0.155 |
| R-free | 0.21994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jis |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.477 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 | 0.085 |
| Number of reflections | 7214 | |
| <I/σ(I)> | 12.5 | 10.5 |
| Completeness [%] | 95.9 | 96.6 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 3-8%(w/v) NaCl, 100mM Na acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
