3AW5

Structure of a multicopper oxidase from the hyperthermophilic archaeon Pyrobaculum aerophilum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-27
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9791, 0.9794, 0.964
Spacegroup name	I 41
Unit cell lengths	103.950, 103.950, 122.083
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.320 - 2.000
R-factor	0.2031
Rwork	0.202
R-free	0.22400
Structure solution method	MAD
RMSD bond length	0.036
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	44415
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sitting drop	5.6	293	PEG4000, ammonium acetate, tri-sodium citrate dihydrate, pH 5.6, sitting drop, temperature 293K
1	sitting drop	5.6	293	PEG4000, ammonium acetate, tri-sodium citrate dihydrate, pH 5.6, sitting drop, temperature 293K
1	sitting drop	5.6	293	PEG4000, ammonium acetate, tri-sodium citrate dihydrate, pH 5.6, sitting drop, temperature 293K