3AV4
Crystal structure of mouse DNA methyltransferase 1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-07 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.900, 0.97894, 0.97954, 1.2454, 1.28220, 1.28309 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 135.780, 97.865, 130.636 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.420 - 2.750 |
| R-factor | 0.2316 |
| Rwork | 0.230 |
| R-free | 0.26710 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 200.000 | 200.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.460 | 2.750 |
| Rmerge | 0.077 | 0.032 | 0.372 |
| Number of reflections | 45792 | ||
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 99.9 | 98.1 | 100 |
| Redundancy | 4.8 | 6.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 297 | PEG 3350, NaCl, TCEP, pH 9.0, vapor diffusion, temperature 297K |
