3AUZ

Crystal structure of Mre11 with manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2010-11-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	101.980, 101.980, 113.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.045 - 3.206
R-factor	0.2235
Rwork	0.219
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3av0
RMSD bond length	0.009
RMSD bond angle	1.250
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	10290
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	60% Tacsimate pH 7.0, 0.5mM manganese chloride , VAPOR DIFFUSION, HANGING DROP, temperature 295K