3AUA
Crystal structure of the quaternary complex-2 of an isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.011, 77.606, 111.736 |
| Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.17457 |
| Rwork | 0.172 |
| R-free | 0.22030 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 46161 | |
| Completeness [%] | 97.2 | 85.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris, 20% PEG8000, 0.3M Ca Acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
