3AU6

DNA polymerase X from Thermus thermophilus HB8 ternary complex with primer/template DNA and ddGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	93
Detector technology	CCD
Collection date	2009-10-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	80.166, 80.166, 268.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.300
R-factor	0.2589
Rwork	0.256
R-free	0.31940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.631
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.820	50.000	3.420
High resolution limit [Å]	3.300	7.100	3.300
Rmerge	0.065	0.027	0.200
Number of reflections	13997
<I/σ(I)>	14.8
Completeness [%]	99.3	97.3	98.1
Redundancy	11.5	11.9	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	50mM Tris-HCl (pH 8.5), 25mM MgSO4 7H2O, 1.8M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K