3AU5
Structure of the human myosin-X MyTH4-FERM cassette
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.99458 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.565, 49.313, 158.407 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 32.680 - 2.550 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3au4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.482 | |
| Number of reflections | 42632 | |
| <I/σ(I)> | 3.29 | |
| Completeness [%] | 97.7 | 92.2 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10% PEG 8000, 5% MPD, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
