3AU2
DNA polymerase X from Thermus thermophilus HB8 complexed with Ca-dGTP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-10-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000, 0.978954, 0.979311 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.189, 53.286, 84.877 |
| Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.400 |
| R-factor | 0.16304 |
| Rwork | 0.162 |
| R-free | 0.18844 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.377 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.940 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.043 | 0.256 |
| Number of reflections | 114469 | |
| <I/σ(I)> | 52.2 | 6.56 |
| Completeness [%] | 98.5 | 97.2 |
| Redundancy | 7.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.2M potassium chloride, 0.01M calcium chloride, 0.005M sodium cacodylate (pH 6.0), 10% PEG 4000 (v/v), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
