3ATQ
Geranylgeranyl Reductase (GGR) from Sulfolobus acidocaldarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.913, 80.935, 105.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.670 - 1.850 |
| R-factor | 0.18945 |
| Rwork | 0.188 |
| R-free | 0.20943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3CGV |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.065 | 0.292 |
| Number of reflections | 46801 | |
| <I/σ(I)> | 28.4 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | 3.5-4.5M Sodium formate, 0.1M Sodium acetate buffer, pH 4.6, VAPOR DIFFUSION, temperature 293K |
